Lateral slit delivery of bone marrow stromal cells enhances regeneration in the decellularized allograft flexor tendon.

ABSTRACT:

Background/objective

Stem cell-based therapy has been applied to accelerate the revitalization of allograft tendon into a viable and functional tendon. Although many authors have proposed different methods to help the seeded stem cell distribution in the decellularized allograft, limited success has been achieved as tendon is a high dense connective tissue. We hypothesized that bone marrow stromal cells (BMSCs), seeded through the lateral slit, can regenerate the decellularized tendon (DCT) graft. The cell proliferation, cell viability, and tendon-specific gene expression are increased with the seeded cell density.

Methods

Eighty-seven flexor digitorum profundus tendons were equally and randomly divided into 6 treatment groups that were seeded with low-density (2 × 10^7<

SUBMITTER: Lu CC 

PROVIDER: S-EPMC6896678 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature