Fibrinolysis protease receptors promote activation of astrocytes to express pro-inflammatory cytokines.

ABSTRACT:

Background

Astrocytes contribute to the crosstalk that generates chronic neuro-inflammation in neurological diseases; however, compared with microglia, astrocytes respond to a more limited continuum of innate immune system stimulants. Recent studies suggest that the fibrinolysis system may regulate inflammation. The goal of this study was to test whether fibrinolysis system components activate astrocytes and if so, elucidate the responsible biochemical pathway.

Methods

Primary cultures of astrocytes and microglia were prepared from neonatal mouse brains. The ability of purified fibrinolysis system proteins to elicit a pro-inflammatory response was determined by measuring expression of the mRNAs encoding tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and chemokine (

SUBMITTER: Pontecorvi P 

PROVIDER: S-EPMC6896679 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature