Tracing the mass flow from glucose and phenylalanine to pinoresinol and its glycosides in Phomopsis sp. XP-8 using stable isotope assisted TOF-MS.

ABSTRACT: Phomopsis sp. XP-8, an endophytic fungus from the bark of Tu-Chung (Eucommia ulmoides Oliv) showed capability to biosynthesize pinoresinol (Pin) and pinoresinol diglucoside (PDG) from glucose (glu) and phenylalanine (Phe). To verify the mass flow in the biosynthesis pathway, [¹³C₆]-labeled glu and [¹³C₆]-labeled Phe were separately fed to the strain as sole substrates and [¹³C₆]-labeled products were detected by ultra-high-performance liquid chromatography-quadrupole time of flight mass spectrometry. As results, [¹³C₆]-labeled Phe was incorporated into [¹³C₆]-cinnamylic acid (Ca) and p-coumaric acid (p-Co), and [¹³C₁₂]-labeled Pin, which revealed that the Pin

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC6897942 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature