Secretome analysis of patient-derived GBM tumor spheres identifies midkine as a potent therapeutic target.

ABSTRACT: Glioblastoma (GBM) is the most lethal primary brain tumor with few treatment options. The survival of glioma-initiating cells (GICs) is one of the major factors contributing to treatment failure. GICs frequently produce and respond to their own growth factors that support cell proliferation and survival. In this study, we aimed to identify critical autocrine factors mediating GIC survival and to evaluate the anti-GBM effect of antagonizing these factors. Proteomic analysis was performed using conditioned media from two different patient-derived GBM tumor spheres under a growth factor-depleted status. Then, the antitumor effects of inhibiting an identified autocrine factor were evaluated by bioinformatic analysis and molecular validation. Proteins secreted by sphere-forming GICs promote cel

SUBMITTER: Han S 

PROVIDER: S-EPMC6897967 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature