Reference gene screening of Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans for quantitative real-time PCR studies.

ABSTRACT: Real-time quantitative PCR studies largely depend on reference genes for the normalization of gene expression. Stable reference genes should be accurately selected in order to obtain reliable results. We here present a study screening commonly used reference genes (TEF1F, α-centractin, Ctsyn1, GAPDH, R6046, APRT and TUB) in the chytrid fungi Batrachochytrium dendrobatidis (Bd) and Batrachochytrium salamandrivorans (Bsal), which cause the lethal amphibian skin disease chytridiomycosis. We evaluated the stability of the reference gene candidates during different growth stages of the fungi, using different statistical software packages: ΔCT, BestKeeper, GeNorm, NormFinder and RefFinder. In order to reflect the in vivo situation, the stability of the candidates was assessed when taking all gro

SUBMITTER: Verbrugghe E 

PROVIDER: S-EPMC6898426 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature