Temperature-dependent regulation of upstream open reading frame translation in S. cerevisiae.

ABSTRACT:

Background

Translation of an mRNA in eukaryotes starts at an AUG codon in most cases, but near-cognate codons (NCCs) such as UUG, ACG, and AUU can also be used as start sites at low levels in Saccharomyces cerevisiae. Initiation from NCCs or AUGs in the 5'-untranslated regions (UTRs) of mRNAs can lead to translation of upstream open reading frames (uORFs) that might regulate expression of the main ORF (mORF). Although there is some circumstantial evidence that the translation of uORFs can be affected by environmental conditions, little is known about how it is affected by changes in growth temperature.

Results

Using reporter assays, we found that changes in growth temperature can affect translation from NCC start sites in yeast cells, suggesting the possibility that gene exp

SUBMITTER: Kulkarni SD 

PROVIDER: S-EPMC6898956 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature