First inter-laboratory comparison of Echinococcus granulosus sensu lato diagnosis in Latin America.

ABSTRACT:

Objective

To compare the performance of polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) tests for diagnosing Echinococcus granulosus in dog feces among national reference laboratories in Argentina, Chile, Peru, and Uruguay.

Methods

National laboratories affiliated with the Ministry of Health/Agriculture of each country exchanged panels of 10 positive/negative samples obtained from their regular national surveillance programs in November 2015 - November 2016. All laboratories applied PCR; two also applied ELISA techniques. Sensitivity and specificity were determined for each laboratory and concordance of results among the laboratories was evaluated by Cohen Kappa coefficient.

Results

Poor concordance (3 of 10 paired comparisons had

SUBMITTER: Jercic MI 

PROVIDER: S-EPMC6898979 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature