Disruption of the Key Ca²⁺ Binding Site in the Selectivity Filter of Neuronal Voltage-Gated Calcium Channels Inhibits Channel Trafficking.

ABSTRACT: Voltage-gated calcium channels are exquisitely Ca²⁺ selective, conferred primarily by four conserved pore-loop glutamate residues contributing to the selectivity filter. There has been little previous work directly measuring whether the trafficking of calcium channels requires their ability to bind Ca²⁺ in the selectivity filter or to conduct Ca²⁺. Here, we examine trafficking of neuronal Ca_V2.1 and 2.2 channels with mutations in their selectivity filter and find reduced trafficking to the cell surface in cell lines. Furthermore, in hippocampal neurons, there is reduced trafficking to the somatic plasma membrane, into neurites, and to presynaptic terminals. However, the Ca_V2.2 selectivity filter mutants are still influenced by auxiliar

SUBMITTER: Meyer JO 

PROVIDER: S-EPMC6899504 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature