Reconstitution and Coupling of DNA Replication and Segregation in a Biomimetic System.

ABSTRACT: A biomimetic system capable of replication and segregation of genetic material constitutes an essential component for the future design of a minimal synthetic cell. Here we have used the simple T7 bacteriophage system and the plasmid-derived ParMRC system to establish in vitro DNA replication and DNA segregation, respectively. These processes were incorporated into biomimetic compartments providing an enclosed reaction space. The functional lifetime of the encapsulated segregation system could be prolonged by equipping it with ATP-regenerating and oxygen-scavenging systems. Finally, we showed that DNA replication and segregation processes could be coupled in vitro by using condensed DNA nanoparticles resulting from DNA replication. ParM spindles extended over tens of micrometers and could

SUBMITTER: Hurtgen D 

PROVIDER: S-EPMC6899551 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature