Comparison of Volumetric and Bead-Based Counting of CD34 Cells by Single-Platform Flow Cytometry.

ABSTRACT:

Background

Over 2,000 people a year in the United Kingdom need a bone marrow or blood stem cell transplant. It is important to accurately quantify the hematopoietic stem cells to predict whether the transplant will be successful in replenishing the immune system. However, they are present at low frequency, which complicates accurate quantification. The current gold standard method is single-platform flow cytometry using internal reference counting beads to determine the concentration of CD34 cells. However, volumetric flow cytometers have the ability to measure the acquisition volume, which removes the need for reference beads for calculation of cell concentrations.

Method

In this study, we compared both methods for calculating CD34 cell concentrations in volumetric cytomete

SUBMITTER: Saraiva L 

PROVIDER: S-EPMC6899615 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature