Arrestin-independent constitutive endocytosis of GPR125/ADGRA3.

ABSTRACT: The orphan receptor GPR125 (ADGRA3) belongs to subgroup III of the adhesion G protein-coupled receptor (aGPCR) family. aGPCRs, also known as class B2 GPCRs, share basic structural and functional properties with other GPCRs. Many of them couple to G proteins and activate G protein-dependent and -independent signaling pathways, but little is known about aGPCR internalization and β-arrestin recruitment. GPR125 was originally described as a spermatogonial stem cell marker and studied for its role in Wnt signaling and cell polarity. Here, using cell-based assays and confocal microscopy, we show that GPR125 is expressed on the cell surface and undergoes constitutive endocytosis in a β-arrestin-independent, but clathrin-dependent manner, as indicated by colocalization with transferrin receptor 1,

SUBMITTER: Spiess K 

PROVIDER: S-EPMC6899654 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature