Unique patterns of glycosylation in immunoglobulin subclass G4-related disease and primary sclerosing cholangitis.

ABSTRACT:

Background and aim

Immunoglobulin subclass G4-related disease (IgG4-RD) is characterized by an abundance of IgG4 antibodies in the serum and tissue. Glycosylation status of antibodies can impact on immune effector functions and disease pathophysiology. We sought to establish glycosylation patterns in a prospective cohort of patients with IgG4-RD and the relationship with disease activity and response to treatment.

Methods

We assessed IgG Fc-tail and Fab-arm glycosylation status in patients with IgG4-RD (n = 22), disease controls with primary sclerosing cholangitis (PSC) (n = 22), and healthy controls (n = 22). Serum IgG and subclasses were quantified using ELISA. Fc and Fab glycosylation were analyzed by mass spectrometry and lectin affinity chromatography, respectively. Dis

SUBMITTER: Culver EL 

PROVIDER: S-EPMC6899843 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature