Actin-spectrin scaffold supports open fenestrae in liver sinusoidal endothelial cells.

ABSTRACT: Fenestrae are open transmembrane pores that are a structural hallmark of healthy liver sinusoidal endothelial cells (LSECs). Their key role is the transport of solutes and macromolecular complexes between the sinusoidal lumen and the space of Disse. To date, the biochemical nature of the cytoskeleton elements that surround the fenestrae and sieve plates in LSECs remain largely elusive. Herein, we took advantage of the latest developments in atomic force imaging and super-resolution fluorescence nanoscopy to define the organization of the supramolecular complex(es) that surround the fenestrae. Our data revealed that spectrin, together with actin, lines the inner cell membrane and provided direct structural support to the membrane-bound pores. We conclusively demonstrated that diamide and io

SUBMITTER: Zapotoczny B 

PROVIDER: S-EPMC6899910 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature