Multi-individual microsatellite identification: A multiple genome approach to microsatellite design (MiMi).

ABSTRACT: Bespoke microsatellite marker panels are increasingly affordable and tractable to researchers and conservationists. The rate of microsatellite discovery is very high within a shotgun genomic data set, but extensive laboratory testing of markers is required for confirmation of amplification and polymorphism. By incorporating shotgun next-generation sequencing data sets from multiple individuals of the same species, we have developed a new method for the optimal design of microsatellite markers. This new tool allows us to increase the rate at which suitable candidate markers are selected by 58% in direct comparisons and facilitate an estimated 16% reduction in costs associated with producing a novel microsatellite panel. Our method enables the visualisation of each microsatellite locus in a

SUBMITTER: Fox G 

PROVIDER: S-EPMC6900094 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature