Identification of histone acetylation markers in human fetal brains and increased H4K5ac expression in neural tube defects.

ABSTRACT:

Background

Neural tube defects (NTDs) are severe common birth defects that result from a failure in neural tube closure (NTC). Our previous study has shown that decreased histone methylation altered the regulation of genes linked to NTC. However, the effect of alterations in histone acetylation in human fetuses with NTDs, which are another functional posttranslation modification, remains elusive. Thus, we aimed to identify acetylation sites and changes in histone in patients with NTDs.

Methods

First, we identified histone acetylation sites between control human embryonic brain tissue and NTDs using Nano-HPLC-MS/MS. Next, we evaluated the level of histone acetylation both groups via western blotting (WB). Finally, we used LC-ESI-MS and WB to compare whether histone H4 acetyla

SUBMITTER: Li D 

PROVIDER: S-EPMC6900389 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature