Circular RNA circ-TSPAN4 promotes lung adenocarcinoma metastasis by upregulating ZEB1 via sponging miR-665.

ABSTRACT:

Background

Cancer metastasis is responsible for 90% of cancer-related deaths. Recently, circular RNA (circRNA) is deemed to be an important regulator of cancer progression. However, little is known about the role of circRNA in the metastasis of lung adenocarcinoma (LUAD). Herein, we investigated the clinical implication and regulatory effect of circ-TSPAN4 (hsa_circ_0020732) in LUAD.

Methods

Gene Expression Omnibus (GEO) database (GSE104854) was used to identify the aberrantly expressed circRNAs in LUAD. The expression levels of circ-TSPAN4, miR-4731-5p, miR-665, and ZEB1 were determined by quantitative reverse transcription PCR (qRT-PCR). The functional experiments were carried out with wound healing and transwell assays. And, the luciferase reporter and RNA pull-down assay

SUBMITTER: Ying X 

PROVIDER: S-EPMC6900391 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature