Contribution of proteasome-catalyzed peptide <i>cis</i>-splicing to viral targeting by CD8⁺ T cells in HIV-1 infection.

ABSTRACT: Peptides generated by proteasome-catalyzed splicing of noncontiguous amino acid sequences have been shown to constitute a source of nontemplated human leukocyte antigen class I (HLA-I) epitopes, but their role in pathogen-specific immunity remains unknown. CD8⁺ T cells are key mediators of HIV type 1 (HIV-1) control, and identification of novel epitopes to enhance targeting of infected cells is a priority for prophylactic and therapeutic strategies. To explore the contribution of proteasome-catalyzed peptide splicing (PCPS) to HIV-1 epitope generation, we developed a broadly applicable mass spectrometry-based discovery workflow that we employed to identify spliced HLA-I-bound peptides on HIV-infected cells. We demonstrate that HIV-1-derived spliced peptides comprise a relatively

SUBMITTER: Paes W 

PROVIDER: S-EPMC6900506 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature