Molybdenum threshold for ecosystem scale alternative vanadium nitrogenase activity in boreal forests.

ABSTRACT: Biological nitrogen fixation (BNF) by microorganisms associated with cryptogamic covers, such as cyanolichens and bryophytes, is a primary source of fixed nitrogen in pristine, high-latitude ecosystems. On land, low molybdenum (Mo) availability has been shown to limit BNF by the most common form of nitrogenase (Nase), which requires Mo in its active site. Vanadium (V) and iron-only Nases have been suggested as viable alternatives to countering Mo limitation of BNF; however, field data supporting this long-standing hypothesis have been lacking. Here, we elucidate the contribution of vanadium nitrogenase (V-Nase) to BNF by cyanolichens across a 600-km latitudinal transect in eastern boreal forests of North America. Widespread V-Nase activity was detected (∼15-50% of total BNF rates), with mo

SUBMITTER: Darnajoux R 

PROVIDER: S-EPMC6900544 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature