Anti-donor MHC Class II Alloantibody Induces Glomerular Injury in Mouse Renal Allografts Subjected to Prolonged Cold Ischemia.

ABSTRACT:

Background

The mechanisms underlying the effects of prolonged cold-ischemia storage on kidney allografts are poorly understood.

Methods

To investigate effects of cold ischemia on donor-reactive immune responses and graft pathology, we used a mouse kidney transplantation model that subjected MHC-mismatched BALB/c kidney allografts to cold-ischemia storage for 0.5 or 6 hours before transplant into C57BL/6 mice.

Results

At day 14 post-transplant, recipients of allografts subjected to 6 versus 0.5 hours of cold-ischemia storage had increased levels of anti-MHC class II (but not class I) donor-specific antibodies, increased donor-reactive T cells, and a significantly higher proportion of transplant glomeruli infiltrated with macrophages. By day 60 post-transplant, allograf

SUBMITTER: Gorbacheva V 

PROVIDER: S-EPMC6900787 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature