Pseudo-chromosome-length genome assembly of a double haploid "Bartlett" pear (Pyrus communis L.).

ABSTRACT:

Background

We report an improved assembly and scaffolding of the European pear (Pyrus communis L.) genome (referred to as BartlettDHv2.0), obtained using a combination of Pacific Biosciences RSII long-read sequencing, Bionano optical mapping, chromatin interaction capture (Hi-C), and genetic mapping. The sample selected for sequencing is a double haploid derived from the same "Bartlett" reference pear that was previously sequenced. Sequencing of di-haploid plants makes assembly more tractable in highly heterozygous species such as P. communis.

Findings

A total of 496.9 Mb corresponding to 97% of the estimated genome size were assembled into 494 scaffolds. Hi-C data and a high-density genetic map allowed us to anchor and orient 87% of the sequence on the 17 pear chromosomes.

SUBMITTER: Linsmith G 

PROVIDER: S-EPMC6901071 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature