The scavenger receptor SCARA1 (CD204) recognizes dead cells through spectrin.

ABSTRACT: Scavenger receptor class A member 1 (SCARA1 or CD204) is an immune receptor highly expressed on macrophages. It forms homotrimers on the cell surface and plays important roles in regulating immune responses via its involvement in multiple pathways. However, both the structure and the functional roles of SCARA1 are not fully understood. Here, we determined the crystal structure of the C-terminal SRCR domain of SCARA1 at 1.8 Å resolution, revealing its Ca²⁺-binding site. Results from cell-based assays revealed that SCARA1 can recognize dead cells, rather than live cells, specifically through its SRCR domain and in a Ca²⁺-dependent manner. Furthermore, by combining MS and biochemical assays, we found that cellular spectrin is the binding target of SCARA1 on dead cells an

SUBMITTER: Cheng C 

PROVIDER: S-EPMC6901321 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature