Decoupling the Effects of the Amyloid Precursor Protein From Amyloid-β Plaques on Axonal Transport Dynamics in the Living Brain.

ABSTRACT: Amyloid precursor protein (APP) is the precursor to Aβ plaques. The cytoplasmic domain of APP mediates attachment of vesicles to molecular motors for axonal transport. In APP-KO mice, transport of Mn²⁺ is decreased. In old transgenic mice expressing mutated human (APP^SwInd) linked to Familial Alzheimer's Disease, with both expression of APP^SwInd and plaques, the rate and destination of Mn²⁺ axonal transport is altered, as detected by time-lapse manganese-enhanced magnetic resonance imaging (MEMRI) of the brain in living mice. To determine the relative contribution of expression of APP^SwInd versus plaque on transport dynamics, we developed a Tet-off system to decouple expression of APP^SwInd from plaque, and then studied hippo

SUBMITTER: Medina CS 

PROVIDER: S-EPMC6901799 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature