Development and Molecular Characterization of Low Phytate Basmati Rice Through Induced Mutagenesis, Hybridization, Backcross, and Marker Assisted Breeding.

ABSTRACT: Breeding low phytate crops is the most viable solution to tackle mineral deficiencies. The objective of the present study was to develop high yielding, low phytate (lpa) basmati rice cultivars. Three homozygous lpa mutants, Lpa5, Lpa9, and Lpa59, were developed through induced mutations (gamma rays ⁶⁰Co) and identified by colorimetric and High Performance Liquid Chromatography (HPLC) analysis. These mutants showed 54%-63% reduction in phytic acid but had poor germination and yield. To improve these traits, hybridization and back cross breeding involving Lpa5, Lpa59, and parent cultivar Super Basmati were performed and F_2:3, F_3:4, BC₁F_2:3, and BC₁F_3:4 generations were developed and screened to target the objective.

SUBMITTER: Qamar ZU 

PROVIDER: S-EPMC6901921 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature