Abiotic Factors Promote Cell Penetrating Peptide Permeability in Enterobacteriaceae Models.

ABSTRACT: Conventionally, the delivery of biomolecules into bacteria for the generation of characterized or functional mutants has relied greatly on horizontal gene transfer techniques. However, the low compatibility of these techniques with novel or hard-to-transform bacteria currently serves as a challenge to the bioengineering field. Here, we explored the use of cell penetrating peptides (CPPs) as an alternative biomolecule delivery approach by investigating the effects of the abiotic factors during CPP permeation. Using the (KFF)₃K-FAM conjugate and Escherichia coli as models, we evaluated four abiotic factors where two of these factors, temperature and solution tonicity, promoted (KFF)₃K-FAM permeation efficiency. Our data show that optimal (KFF)₃K-FAM pe

SUBMITTER: Toyohara D 

PROVIDER: S-EPMC6902036 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature