The Cell Cycle G2/M Block Is an Indicator of Cellular Radiosensitivity.

ABSTRACT:

Background

Determination of the radiosensitivity of a specific tumor is essential to its precision tumor radiotherapy, but the measurement of cellular radiosensitivity with a routine colony forming assay is both labor- and time-consuming. An alternative option allowing rapid and precise prediction of radiosensitivity is necessary.

Methods

In this study, we exposed 4 in vitro cultured cell lines to various doses of X-rays or carbon ions and then measured their radiosensitivities with a routine colony-forming assay, and monitored the kinetics of cell cycle distribution with routine propidium iodine staining and flow cytometry.

Results

Based on the results, we correlated cellular radiosensitivity with a dynamic assay of cell cycle distribution, specifically, the negative

SUBMITTER: Liu C 

PROVIDER: S-EPMC6902394 | biostudies-literature | 2019 Oct-Dec

REPOSITORIES: biostudies-literature