Diazaquinomycin Biosynthetic Gene Clusters from Marine and Freshwater Actinomycetes.

ABSTRACT: Tuberculosis is an infectious disease of global concern. Members of the diazaquinomycin (DAQ) class of natural products have shown potent and selective activity against drug-resistant Mycobacterium tuberculosis. However, poor solubility has prevented further development of this compound class. Understanding DAQ biosynthesis may provide a viable route for the generation of derivatives with improved properties. We have sequenced the genomes of two actinomycete bacteria that produce distinct DAQ derivatives. While software tools for automated biosynthetic gene cluster (BGC) prediction failed to detect DAQ BGCs, comparative genomics using MAUVE alignment led to the identification of putative BGCs in the marine Streptomyces sp. F001 and in the freshwater Micromonospora sp. B006. Deletion of the

SUBMITTER: Braesel J 

PROVIDER: S-EPMC6902439 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature