M⁶A mRNA methylation initiated by METTL3 directly promotes YAP translation and increases YAP activity by regulating the MALAT1-miR-1914-3p-YAP axis to induce NSCLC drug resistance and metastasis.

ABSTRACT:

Background

METTL3 is an RNA methyltransferase that mediates m⁶A modification and is implicated in mRNA biogenesis, decay, and translation. However, the biomechanism through which METTL3 regulates MALAT1-miR-1914-3p-YAP axis activity to induce NSCLC drug resistance and metastasis is not very clear.

Methods

The expression of mRNA was analyzed by qPCR assays. Protein levels were analyzed by western blotting and immunofluorescent staining. Cellular proliferation was detected by CCK8 assays. Cell migration and invasion were analyzed by wound healing and transwell assays, respectively. Promoter activities and gene transcription were analyzed by luciferase reporter assays. Finally, m⁶A modification was analyzed by MeRIP.

Results

METTL3 increased the m

SUBMITTER: Jin D 

PROVIDER: S-EPMC6902496 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature