A new method for quantitative detection of Lactobacillus casei based on casx gene and its application.

ABSTRACT:

Background

The traditional method of bacterial identification based on 16S rRNA is a widely used and very effective detection method, but this method still has some deficiencies, especially in the identification of closely related strains. A high homology with little differences is mostly observed in the 16S sequence of closely related bacteria, which results in difficulty to distinguish them by 16S rRNA-based detection method. In order to develop a rapid and accurate method of bacterial identification, we studied the possibility of identifying bacteria with other characteristic fragments without the use of 16S rRNA as detection targets.

Results

We analyzed the potential of using cas (CRISPR-associated proteins) gene as a target for bacteria detection. We found that certain

SUBMITTER: Pang X 

PROVIDER: S-EPMC6902566 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature