Proteome changes of porcine follicular fluid during follicle development.

ABSTRACT:

Background

Ovarian follicular fluid influences follicle and oocyte growth, but the fluctuation of its protein content during folliculogenesis has not been comprehensively analyzed. Here we used a shotgun approach and bioinformatics analyses to investigate and compare the proteomes of porcine follicular fluid (pFF) obtained from small (< 4 mm), medium (4-6 mm) and large (> 6-12 mm) follicles.

Results

Follicular fluid samples containing highest estrogen levels were selected as non-atretic from small (SNA: 26.1 ± 15 ng/mL), medium (MNA: 162 ± 54 ng/mL), and large (LNA: 290 ± 37 ng/mL) follicles for proteomic analyses. We detected 1627, 1699, and 1756 proteins in SNA, MNA, and LNA samples, respectively. Nearly 60-63% of total proteins were specific to each sample, 11-13% were sh

SUBMITTER: Paes VM 

PROVIDER: S-EPMC6902611 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature