Noninvasive longitudinal quantification of β-cell mass with [¹¹¹In]-labeled exendin-4.

ABSTRACT: Currently, quantifying β-cell mass (BCM) requires harvesting the pancreas. In this study, we investigated a potential noninvasive method to quantify BCM changes longitudinally using [Lys¹²(¹¹¹In-BnDTPA-Ahx)]exendin-4 ([¹¹¹In]-Ex4) and single-photon emission computed tomography (SPECT). We used autoradiography and transgenic mice expressing green fluorescent protein under the control of mouse insulin 1 gene promotor to evaluate the specificity of [¹¹¹In]-Ex4 toward β cells. Using nonobese diabetic (NOD) mice, we injected [¹¹¹In]-Ex4 (3.0 MBq) intravenously and performed SPECT 30 min later, repeating this at a 2-wk interval. After the second scan, we harvested the pancreas and calculated BCM from immunohistochemically stained pancreatic

SUBMITTER: Fujita N 

PROVIDER: S-EPMC6902711 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature