Automated glycan assembly of arabinomannan oligosaccharides from Mycobacterium tuberculosis.

ABSTRACT: Arabinomannan (AM) polysaccharides are clinical biomarkers for Mycobacterium tuberculosis (MTB) infections due to their roles in the interaction with host cells and interference with macrophage activation. Collections of defined AM oligosaccharides can help to improve the understanding of these polysaccharides and the development of novel therapeutical and diagnostic agents. Automated glycan assembly (AGA) was employed to prepare the core structure of AM from MTB, containing ?-(1,6)-Man, ?-(1,5)-Ara, and ?-(1,2)-Man linkages. The introduction of a capping step after each glycosylation and further optimized reaction conditions allowed for the synthesis of a series of oligosaccharides, ranging from hexa- to branched dodecasaccharides.

SUBMITTER: Pardo-Vargas A 

PROVIDER: S-EPMC6902893 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature