Sox9-Meis1 Inactivation Is Required for Adipogenesis, Advancing Pref-1⁺ to PDGFRα⁺ Cells.

ABSTRACT: Adipocytes arise from the commitment and differentiation of adipose precursors in white adipose tissue (WAT). In studying adipogenesis, precursor markers, including Pref-1 and PDGFRα, are used to isolate precursors from stromal vascular fractions of WAT, but the relation among the markers is not known. Here, we used the Pref-1 promoter-rtTA system in mice for labeling Pref-1⁺ cells and for inducible inactivation of the Pref-1 target Sox9. We show the requirement of Sox9 for the maintenance of Pref-1⁺ proliferative, early precursors. Upon Sox9 inactivation, these Pref-1⁺ cells become PDGFRα⁺ cells that express early adipogenic markers. Thus, we show that Pref-1⁺ cells precede PDGFRα⁺ cells in the adipogenic pathway and that S

SUBMITTER: Gulyaeva O 

PROVIDER: S-EPMC6903418 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature