Disruption of transcription-translation coordination in Escherichia coli leads to premature transcriptional termination.

ABSTRACT: Tight coordination between transcription and translation is crucial to maintaining the integrity of gene expression in bacteria, yet how bacteria manage to coordinate these two processes remains unclear. Possible direct physical coupling between the RNA polymerase and ribosome has been thoroughly investigated in recent years. Here, we quantitatively characterize the transcriptional kinetics of Escherichia coli under different growth conditions. Transcriptional and translational elongation remain coordinated under various nutrient conditions, as previously reported. However, transcriptional elongation was not affected under antibiotics that slowed down translational elongation. This result was also found by introducing nonsense mutation that completely dissociated transcription from transla

SUBMITTER: Zhu M 

PROVIDER: S-EPMC6903697 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature