BIN overlap confirms transcontinental distribution of pest aphids (Hemiptera: Aphididae).

ABSTRACT: DNA barcoding is highly effective for identifying specimens once a reference sequence library is available for the species assemblage targeted for analysis. Despite the great need for an improved capacity to identify the insect pests of crops, the use of DNA barcoding is constrained by the lack of a well-parameterized reference library. The current study begins to address this limitation by developing a DNA barcode reference library for the pest aphids of Pakistan. It also examines the affinities of these species with conspecific populations from other geographic regions based on both conventional taxonomy and Barcode Index Numbers (BINs). A total of 809 aphids were collected from a range of plant species at sites across Pakistan. Morphological study and DNA barcoding allowed 774 specimens

SUBMITTER: Naseem MT 

PROVIDER: S-EPMC6903727 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature