Global Analysis of Enhancer Targets Reveals Convergent Enhancer-Driven Regulatory Modules.

ABSTRACT: Single-cell screens enable high-throughput functional assessment of enhancers in their endogenous genomic context. However, the design of current studies limits their application to identifying the primary gene targets of enhancers. Here, we improve the experimental and computational parameters of single-cell enhancer screens to identify the secondary gene targets of enhancers. Our analysis of >500 putative enhancers in K562 cells reveals an interwoven enhancer-driven gene regulatory network. We find that enhancers from distinct genomic loci converge to modulate the expression of common sub-modules, including the α- and β-globin loci, by directly regulating transcription factors. Our analysis suggests that several genetic variants associated with myeloid blood cell traits alter the activit

SUBMITTER: Xie S 

PROVIDER: S-EPMC6904118 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature