Long-Term Culture Captures Injury-Repair Cycles of Colonic Stem Cells.

ABSTRACT: The colonic epithelium can undergo multiple rounds of damage and repair, often in response to excessive inflammation. The responsive stem cell that mediates this process is unclear, in part because of a lack of in vitro models that recapitulate key epithelial changes that occur in vivo during damage and repair. Here, we identify a Hopx⁺ colitis-associated regenerative stem cell (CARSC) population that functionally contributes to mucosal repair in mouse models of colitis. Hopx⁺ CARSCs, enriched for fetal-like markers, transiently arose from hypertrophic crypts known to facilitate regeneration. Importantly, we established a long-term, self-organizing two-dimensional (2D) epithelial monolayer system to model the regenerative properties and responses of Hopx⁺ C

SUBMITTER: Wang Y 

PROVIDER: S-EPMC6904908 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature