Single-Stranded DNA Translocation Recordings through Solid-State Nanopores on Glass Chips at 10 MHz Measurement Bandwidth.

ABSTRACT: Accurate and low-cost analysis of biomolecules is important for many applications. This work seeks to further improve the measurement bandwidths achievable with solid-state nanopores, which have emerged as an important platform for this analysis. We report single-stranded DNA translocation recordings at a bandwidth of 10 MHz copolymers of 80 (C₂₀A₂₀C₂₀A₂₀), 90 (C₃₀A₃₀C₃₀), and 200 (C₅₀A₅₀C₅₀A₅₀) nucleotides through Si nanopores with effective diameters of 1.4-2.1 nm and effective membrane thicknesses 0.5-8.9 nm. By optimizing glass chips with thin nanopores and by integrating them with custom-designed amplifiers based on complementary metal-oxide-semiconductor technology

SUBMITTER: Chien CC 

PROVIDER: S-EPMC6904932 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature