A new vector system for targeted integration and overexpression of genes in the crop pathogen Fusarium solani.

ABSTRACT:

Background

Besides their ability to produce several interesting bioactive secondary metabolites, members of the Fusarium solani species complex comprise important pathogens of plants and humans. One of the major obstacles in understanding the biology of this species complex is the lack of efficient molecular tools for genetic manipulation.

Results

To remove this obstacle we here report the development of a reliable system where the vectors are generated through yeast recombinational cloning and inserted into a specific site in F. solani through Agrobacterium tumefaciens-mediated transformation. As proof-of-concept, the enhanced yellow fluorescent protein (eYFP) was inserted in a non-coding genomic position of F. solani and subsequent analyses show

SUBMITTER: Nielsen MR 

PROVIDER: S-EPMC6905090 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature