Differential neuronal reprogramming induced by NeuroD1 from astrocytes in grey matter versus white matter.

ABSTRACT: A new technology called in vivo glia-to-neuron conversion has emerged in recent years as a promising next generation therapy for neural regeneration and repair. This is achieved through reprogramming endogenous glial cells into neurons in the central nervous system through ectopically expressing neural transcriptional factors in glial cells. Previous studies have been focusing on glial cells in the grey matter such as the cortex and striatum, but whether glial cells in the white matter can be reprogrammed or not is unknown. To address this fundamental question, we express NeuroD1 in the astrocytes of both grey matter (cortex and striatum) and white matter (corpus callosum) to investigate the conversion efficiency, neuronal subtypes, and electrophysiological features of the converted neuron

SUBMITTER: Liu MH 

PROVIDER: S-EPMC6905344 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature