Establishment of normative ranges of the healthy human immune system with comprehensive polychromatic flow cytometry profiling.

ABSTRACT: Existing normative flow cytometry data have several limitations including small sample sizes, incompletely described study populations, variable flow cytometry methodology, and limited depth for defining lymphocyte subpopulations. To overcome these issues, we defined high-dimensional flow cytometry reference ranges for the healthy human immune system using Human Immunology Project Consortium methodologies after carefully screening 127 subjects deemed healthy through clinical and laboratory testing. We enrolled subjects in the following age cohorts: 18-29 years, 30-39, 40-49, and 50-66 and enrolled cohorts to ensure an even gender distribution and at least 30% non-Caucasians. From peripheral blood mononuclear cells, flow cytometry reference ranges were defined for >50 immune subsets includi

SUBMITTER: Yi JS 

PROVIDER: S-EPMC6905525 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature