Prolonged ovarian storage of mature Drosophila oocytes dramatically increases meiotic spindle instability.

ABSTRACT: Human oocytes frequently generate aneuploid embryos that subsequently miscarry. In contrast, Drosophila oocytes from outbred laboratory stocks develop fully regardless of maternal age. Since mature Drosophila oocytes are not extensively stored in the ovary under laboratory conditions like they are in the wild, we developed a system to investigate how storage affects oocyte quality. The developmental capacity of stored mature Drosophila oocytes decays in a precise manner over 14 days at 25°C. These oocytes are transcriptionally inactive and persist using ongoing translation of stored mRNAs. Ribosome profiling revealed a progressive 2.3-fold decline in average translational efficiency during storage that correlates with oocyte functional decay. Although normal bipolar meiotic spindles predom

SUBMITTER: Greenblatt EJ 

PROVIDER: S-EPMC6905857 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature