Individual brain organoids reproducibly form cell diversity of the human cerebral cortex.

ABSTRACT: Experimental models of the human brain are needed for basic understanding of its development and disease¹. Human brain organoids hold unprecedented promise for this purpose; however, they are plagued by high organoid-to-organoid variability^2,3. This has raised doubts as to whether developmental processes of the human brain can occur outside the context of embryogenesis with a degree of reproducibility that is comparable to the endogenous tissue. Here we show that an organoid model of the dorsal forebrain can reliably generate a rich diversity of cell types appropriate for the human cerebral cortex. We performed single-cell RNA-sequencing analysis of 166,242 cells isolated from 21 individual organoids, finding that 95% of the organoids generate a virtually indistinguis

SUBMITTER: Velasco S 

PROVIDER: S-EPMC6906116 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature