Survival of aging CD264⁺ and CD264^- populations of human bone marrow mesenchymal stem cells is independent of colony-forming efficiency.

ABSTRACT: In vivo mesenchymal stem cell (MSC) survival is relevant to therapeutic applications requiring engraftment and potentially to nonengraftment applications as well. MSCs are a mixture of progenitors at different stages of cellular aging, but the contribution of this heterogeneity to the survival of MSC implants is unknown. Here, we employ a biomarker of cellular aging, the decoy TRAIL receptor CD264, to compare the survival kinetics of two cell populations in human bone marrow MSC (hBM-MSC) cultures. Sorted CD264⁺ hBM-MSCs from two age-matched donors have elevated β-galactosidase activity, decreased differentiation potential and form in vitro colonies inefficiently relative to CD264^- hBM-MSCs. Counterintuitive to their aging phenotype, CD264⁺ hBM-MSCs exhibit

SUBMITTER: Madsen SD 

PROVIDER: S-EPMC6906265 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature