The SETD6 Methyltransferase Plays an Essential Role in Hippocampus-Dependent Memory Formation.

ABSTRACT:

Background

Epigenetic mechanisms are critical for hippocampus-dependent memory formation. Building on previous studies that implicate the N-lysine methyltransferase SETD6 in the activation of nuclear factor-κB RELA (also known as transcription factor p65) as an epigenetic recruiter, we hypothesized that SETD6 is a key player in the epigenetic control of long-term memory.

Methods

Using a series of molecular, biochemical, imaging, electrophysiological, and behavioral experiments, we interrogated the effects of short interfering RNA-mediated knockdown of Setd6 in the rat dorsal hippocampus during memory consolidation.

Results

Our findings demonstrate that SETD6 is necessary for memory-related nuclear factor-κB RELA methylation at lysine 310 and associated increases in H3

SUBMITTER: Webb WM 

PROVIDER: S-EPMC6906268 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature