Crowdsourcing and the feasibility of manual gene annotation: A pilot study in the nematode Pristionchus pacificus.

ABSTRACT: Nematodes such as Caenorhabditis elegans are powerful systems to study basically all aspects of biology. Their species richness together with tremendous genetic knowledge from C. elegans facilitate the evolutionary study of biological functions using reverse genetics. However, the ability to identify orthologs of candidate genes in other species can be hampered by erroneous gene annotations. To improve gene annotation in the nematode model organism Pristionchus pacificus, we performed a genome-wide screen for C. elegans genes with potentially incorrectly annotated P. pacificus orthologs. We initiated a community-based project to manually inspect more than two thousand candidate loci and to propose new gene models based on recently generated Iso-seq and RNA-seq data. In most cases, misannot

SUBMITTER: Rodelsperger C 

PROVIDER: S-EPMC6906410 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature