The fitness cost of mismatch repair mutators in Saccharomyces cerevisiae: partitioning the mutational load.

ABSTRACT: Mutational load is the depression in a population's mean fitness that results from the continual influx of deleterious mutations. Here, we directly estimate the mutational load in a population of haploid Saccharomyces cerevisiae that are deficient for mismatch repair. We partition the load in haploids into two components. To estimate the load due to nonlethal mutations, we measure the competitive fitness of hundreds of randomly selected clones from both mismatch-repair-deficient and -proficient populations. Computation of the mean clone fitness for the mismatch-repair-deficient strain permits an estimation of the nonlethal load, and the histogram of fitness provides an interesting visualization of a loaded population. In a separate experiment, in order to estimate the load due to lethal mu

SUBMITTER: Galeota-Sprung B 

PROVIDER: S-EPMC6906417 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature