The impact of particulate matter (PM2.5) on skin barrier revealed by transcriptome analysis: Focusing on cholesterol metabolism.

ABSTRACT: Accumulating evidence suggests that particulate matter (PM2.5), as a major air pollutant, imposes a certain degree of destruction and toxicity to the skin. It particularly impairs the structure and function of the epidermis. To study the impact of PM2.5 on the skin, transcriptome analysis was performed on PM2.5-exposed human primary keratinocytes. Functional annotation analysis demonstrates that PM2.5 significantly up-regulates cholesterol-metabolism-related genes. Via lipid extraction from PM2.5 treated three-dimensional epidermis tissue model (3D-ETM) and subsequent characterization via mass spectrometry, it was confirmed that PM2.5 significantly increases epidermal cholesterol levels in vitro. Conversely, the amount of squalene in 3D-ETM was significantly reduced by PM2.5. Intere

SUBMITTER: Liao Z 

PROVIDER: S-EPMC6906712 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature