Global Assessment of Mycobacterium avium subsp. hominissuis Genetic Requirement for Growth and Virulence.

ABSTRACT: Nontuberculous mycobacterial infections caused by the opportunistic pathogen Mycobacterium avium subsp. hominissuis (MAH) are currently receiving renewed attention due to increased incidence combined with difficult treatment. Insights into the disease-causing mechanisms of this species have been hampered by difficulties in genetic manipulation of the bacteria. Here, we identified and sequenced a highly transformable, virulent MAH clinical isolate susceptible to high-density transposon mutagenesis, facilitating global gene disruption and subsequent investigation of MAH gene function. By transposon insertion sequencing (TnSeq) of this strain, we defined the MAH genome-wide genetic requirement for virulence and in vitro growth and organized ∼3,500 identified transposon mu

SUBMITTER: Dragset MS 

PROVIDER: S-EPMC6906737 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature