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Extraction of Polysaccharide from Dendrobium nobile Lindl. by Subcritical Water Extraction.

ABSTRACT: Subcritical water extraction (SWE) uses hot compressed water as an effective solvent for both polar and nonpolar compounds and has been developed as an environmentally benign extraction technology for natural materials. Polysaccharides as one of the main ingredients in Dendrobium plants showed obvious biological activity. Thus, SWE of polysaccharides obtained from Dendrobium nobile Lindl. was investigated in this work. The response surface methodology (RSM) was combined with a Box-Behnken design to evaluate the influence that the three independent variables had on the response. The optimal extraction conditions (determined via RSM) were 129.83 °C extraction temperature, 16.71 min extraction time, and 1.12 MPa extraction pressure. The maximum predicted polysaccharide yield was 20.67%, which corresponded well with the experiential extraction (21.88%). The polysaccharides obtained from either the stirring extraction, refluxing extraction, ultrasound extraction, or SWE methods were compared, and the extraction processes were modeled. The molecular weight, monosaccharide composition, and antioxidative activities of the polysaccharides were analyzed.

SUBMITTER: Liu J

PROVIDER: S-EPMC6906767 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Extraction of Polysaccharide from Dendrobium nobile Lindl. by Subcritical Water Extraction.

Liu Jing J Li Yucheng Y Liu Wangqu W Qi Qi Q Hu Xin X Li Siya S Lei Jiandu J Rong Long L

ACS omega 20191127 24

Subcritical water extraction (SWE) uses hot compressed water as an effective solvent for both polar and nonpolar compounds and has been developed as an environmentally benign extraction technology for natural materials. Polysaccharides as one of the main ingredients in Dendrobium plants showed obvious biological activity. Thus, SWE of polysaccharides obtained from Dendrobium nobile Lindl. was investigated in this work. The response surface methodology (RSM) was combined with a Box- ...[more]

PMID: 31858044

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Project description:ObjectivesDendrobium catenatum Lindl. (DH) is a Chinese herbal medicine, which is often used to make tea to improve immunity in China. Rumor has it that DH has a protective effect against cardiovascular disease. However, it is not clear how DH can prevent cardiovascular disease, such as atherosclerosis (AS). Therefore, the purpose of this study is to study whether DH can prevent AS and the underlying mechanisms.MethodsZebrafish larvae were fed with high-cholesterol diet (HCD) to establish a zebrafish AS model. Then, we used DH water extracts (DHWE) to pretreat AS zebrafish. The plaque formation was detected by HE, EVG, and oil red O staining. Neutrophil and macrophage counts were calculated to evaluate the inflammation level. Reactive oxygen species (ROS) activity, malondialdehyde (MDA) content, and superoxide dismutase (SOD) activity in zebrafish were measured to reflect oxidative stress. The cholesterol accumulation and the levels of lipid, triglyceride (TG), and total cholesterol (TC) were measured to reflect lipid metabolism disorder. Then, parallel flow chamber was utilized to establish a low shear stress- (LSS-) induced endothelial cell (EC) dysfunction model. EA.hy926 cells were exposed to LSS (3 dyn/cm2) for 30 min and treated with DHWE. The levels of ROS, SOD, MDA, glutathione (GSH), and glutathiol (GSSG) in EA.hy926 cells were analysed to determine oxidative stress. The release of nitric oxide (NO), endothelin-1 (ET-1), and epoprostenol (PGI2) in EA.hy926 cells was measured to reflect EC dysfunction. The mRNA expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in EA.hy926 cells was detected to reflect EC dysfunction inflammation.ResultsThe results showed that DHWE significantly reduced cholesterol accumulation and macrophage infiltration in early AS. Finally, DHWE significantly alleviate the lipid metabolism disorder, oxidative stress, and inflammation to reduce the plaque formation of AS zebrafish larval model. Meanwhile, we also found that DHWE significantly improved LSS-induced EC dysfunction and oxidative stress in vitro.ConclusionOur results indicate that DHWE could be used as a prevention method to prevent AS.

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Extraction of Polysaccharide from Dendrobium nobile Lindl. by Subcritical Water Extraction.

Publications

Extraction of Polysaccharide from <i>Dendrobium nobile</i> Lindl. by Subcritical Water Extraction.

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