Transcriptomic analyses reveal physiological changes in sweet orange roots affected by citrus blight.

ABSTRACT:

Background

Citrus blight is a very important progressive decline disease of commercial citrus. The etiology is unknown, although the disease can be transmitted by root grafts, suggesting a viral etiology. Diagnosis is made by demonstrating physical blockage of xylem cells that prevents the movement of water. This test was used to identify symptomatic trees from four commercial groves in Florida. Total RNA extracts of phloem-enriched scaffold root tissues were prepared from seven trees that failed to take up water and from one healthy tree. These RNA extracts were used for transcriptomic analyses using paired end RNA-Seq from an Illumina 2500 system. The expression of transcripts annotated as polyprotein of citrus endogenous pararetrovirus were estimated by both RT-qPCR and RNA-Seq.

SUBMITTER: Fu S 

PROVIDER: S-EPMC6907255 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature